The star has given the full amount of his £25,000 award cash to the trust for venues, which works to protect the UK's independent live music spots.
Fender was presented with the prize on 16 October as his third album People Watching was declared the outstanding release of the last 12 months.
Fender has opted to give the cash to the trust, in recognition of the crucial part small stages played in his beginning days.
"I wouldn't be doing my current career if it hadn't been for all the performances I played around the region, and further afield, when I was starting out," said Fender. "These spaces are renowned, but they are facing challenges."
Since the start of 2023, at least 150 of these spaces have permanently closed – approximately 16 percent of the entire UK sector.
Recently, major artists including Pulp, Coldplay, Katy Perry and Sheeran have all aided the MVT in its efforts to support the industry, by including a modest fee to their ticket prices, which supports smaller concert halls.
Sam Fender also took part, collecting over £100k on his live tour to support numerous grassroots venues across Britain.
The money supported locations that were confronting possible closure due to difficulties caused by water damage, fire incidents and family tragedies, as well as licensing issues, conflicts and noise complaints.
Some locations received funding support in upgrading infrastructure and sound and lighting systems that positively impacted artists and concertgoers.
"The notion that revenue from performances in large arenas helps the local stages, where it kicks off for musicians like me, is just sensible," the star has commented.
The musician is not the first artist to contribute his award winnings to worthy causes.
When they received the award in 1996 for their record their work, lead singer Jarvis Cocker announced that the group would contribute their prize money to the non-profit War Child.
That year, rapper and singer she split her award across multiple charities, such as the NSPCC and a Sickle Cell organization.
"I also contributed a grand to the center, to their gymnastics class, because I did gymnastics when I was growing up and they needed better apparatus," the artist told the media.
Previously, they gave their winnings to the youth organization that nurtured their group, as well as other local music groups.
And winners that year they donated their winnings to a charity for MS after a acquaintance was diagnosed the disease.
"The victory was sufficient," remarked vocalist Small. "The money was the extra but we didn't require the extra, because we had the cake. So our winning touched the lives of others."
Last week, the Music Venues Trust announced it had rescued a couple of local spots in south-east England, by bringing them into local control.
One venue in that area and the second in that location were bought under the community ownership initiative, which is supported by a national body and music fans who acquire "ownership units" in the venues.
